Proximity-dependent Biotin Identification (BioID) Service for Protein-Protein Interactions (PPIs)

High-level Schematic of the BioID Process

Overview

BioID is a powerful tool for the in vivo identification of both stable and transient protein interactions. Developed by Dr. Kyle Roux (references), the method fuses a biotin ligase (BirA) to the protein of interest to allow for selective biotinylation of proteins that either interact with the protein of interest or that enter into the labeling radius of the BirA. Subsequent affinity purification and LCMS analysis allow for identification of the biotinylated proteins providing researchers with insights into protein interactions.

QuarryBio is able to provide services for our clients at any stage of the BioID process. Please contact us if you would like to talk about your project and how we might be able to help.

BioID with QuarryBio

QuarryBio is now offering BioID services to for clients. Working closely with BioFront Technologies and Dr. Roux, QuarryBio has developed the capabilities to perform all aspects of BioID for our clients, including:

  • Plasmid construction

  • Stable cell line generation for both the BirA and control cell lines

  • Biotinylation and pull down

  • LCMS analysis

  • Data workup

References

Roux, K. J., Kim, D. I., Burke, B., & May, D. G. (2018). BioID: A screen for protein-protein interactions. Current Protocols in Protein Science, 91, 19.23.1–19.23.15. doi: 10.1002/cpps.51

Sears, M. S., May, D. G., Roux, K. J. (2019) BioID as a Tool for Protein-Proximity Labeling in Living Cells. Methods in Molecular Biology, 2012, 299-313. Doi 10.1007/978-1-4939-9546-2_15.